Cellular Response and Adaptation to Heat-Induced Stress by Role of Heat Shock Proteins (HSPs)
Department of Biology, College of Education for Pure Science, University of Kirkuk, Iraq.
Research Article
International Journal of Biological and Pharmaceutical Sciences Archive, 2025, 10(02), 161-169.
Article DOI: 10.53771/ijbpsa.2025.10.2.0087
Publication history:
Received 18 October 2025; revised on 26 November 2025; accepted on 29 November 2025
Abstract:
This paper set out to examine the use of heat shock proteins (HSPs) in the cellular response to heat-induced stress in mammalian cells in controlled in vitro models. There was an exposure to high temperature (42C) of cultured cells, but this exposure was brief and then a normal physiological temperature (recovery) period. Changes arising in cell viability, morphology, and expression of essential heat shock proteins, specifically, HSP70 and HSP90 were evaluated by the experimental design.
Findings have shown that the heat stress had a transient effect on cell viability and morphological changes were observed, such as partial cell rounding and detachment. Nevertheless, a vast majority of cells recovered substantially upon restoration of normal conditions and indicated the capacity of mammalian cells to possess adaptation to temporary thermal stress. Both Hsp70 and Hsp90 exhibited a great rise in response to heat exposure, however, Hsp70 exhibited better and longer response. These findings confirm the importance of HSPs as molecular chaperones to control protein structure, prevent aggregation and facilitate recovery responses following stress.
the article shows the paramount role of the heat shock proteins, particularly HSP70, in ensuring cellular integrity and facilitating adaptive responses of the cell to thermal stress. The fact that these protective mechanisms are stimulated points out the importance of the role of HSPs as the main mechanisms of cellular homeostasis and stress resistance.
Keywords:
Heat-Induced; HSP70; Heat Shock Proteins; HSP90
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Copyright © 2025 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0