Detection of total flavonoid, antioxidant activity and HPLC analysis of methanolic extract of Silybum marianum seeds
1 Collage of Biotechnology, Al-Nahrain University, Baghdad, Iraq.
2 Ministry of science and technology, Baghdad, Iraq.
Research Article
International Journal of Biological and Pharmaceutical Sciences Archive, 2022, 03(02), 102–107.
Article DOI: 10.53771/ijbpsa.2022.3.2.0034
Publication history:
Received on 03 March 2022; revised on 17 April 2022; accepted on 17 April 2022
Abstract:
Silybum marianum considered as an ancient herb promising plants. In this work, fhese study was design to assess total flavonoids, anti-oxidant and HPLC analysis of Silybum marianum plant extract by methanol in vitro. Total flavonoids had been measured in the extract that about 201.6667±1.52753 mg/ml. anti-oxidant activity of methanolic plant extract Silybum marianum in vitro was estimated by assesment of reductive ability, in concentrations that be tested (0.02, 0.04, 0.08, 0.16, 0.32 and 0.64 mg/ml) , the absorbance had been increased significantly , results of HPLC analysis of plant contain (Ascorbic acid ,Gallic acid Catechin, Rutin, Quercetin, Apigeninand Kaemp ferol) in concentration (0.839, 0.0918, 0.628, 2.989, 0.010, 0.0680, 0.228) ppm respectivily.
Keywords:
Silybum marianum; HPLC; Total flavonoids; anti-oxidant activity; Ascorbic acid
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