A phytochemical analysis, isolation and anti-fungal investigation of the Batis maritima shrub (Crabgrass)
1 Division of Natural Science, University of Guyana Berbice Campus, Tain, Guyana.
2 Faculty of Agriculture and Forestry, University of Guyana Berbice Campus, Tain, Guyana.
Research Article
International Journal of Biological and Pharmaceutical Sciences Archive, 2021, 01(02), 248–256.
Article DOI: 10.30574/ijbpsa.2021.1.2.0053
Publication history:
Received on 29 April 2021; revised on 06 June 2021; accepted on 10 June 2021
Abstract:
Batis maritima (or Saltwort, Turtle weed and Crabgrass) is halophyte that grows along the coast of many tropical and subtropical regions. It is widely considered a weed as it not widely used. The few documented cases of its uses, however, strongly suggest that it possesses antifungal properties. Crude alcoholic extracts B. maritima of varying concentrations were tested against five fungal species: Aspergillus niger, Aspergillus flavus, Malassezia sp., Pyricularia oryzae, and Rhizopus sp. The Well-diffusion method was used to determine the Zones of Inhibition with respect to the fungi tested. The crude extracts proved especially effective against P. oryzae, with an inhibition zone of 26.3 ± 0.95 mm with the 1% solution and maximum inhibition with higher concentrations tested. Conversely, no inhibition zone was observed against Rhizopus sp.
The crude alcoholic extract was also subjected to Column Chromatography and six compounds were isolated. Solutions of four of these were tested against the mentioned fungi. Antifungal activity was confirmed in all four of the isolates. A phytochemical screening was also done to identify the active secondary metabolites present in the crude extract. Those confirmed were glycosides, alkaloids, flavonoids, saponins, sterols, tannins and terpenoids.
The aim of this research was therefore to determine the antifungal properties of alcoholic extracts of the B. maritima as well as to perform a phytochemical screening on the crude extracts.
Keywords:
Batis maritima; Saltwort; Turtle weed; Crabgrass; Antifungal activities; Phytochemical screening
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