Histological and proximate analysis of ash produced from sesame, palm fruit and Parkia on albino rats
1 Department of Human Nutrition and Dietetics, Faculty of Food Technology University of Agriculture Krakow, Poland.
2 Departments of Nutrition and Dietetics, Faculty of Agriculture, Nasarawa State University Keffi, Shabu-Lafia.
Research Article
International Journal of Biological and Pharmaceutical Sciences Archive, 2021, 02(02), 026–030.
Article DOI: 10.53771/ijbpsa.2021.2.2.0077
Publication history:
Received on 01 September 2021; revised on 30 October 2021; accepted on 01 November 2021
Abstract:
The customary puerperal usage of Natron consumption has been identified as one of the risk factors in the cause of high blood pressure in human as well as kidney diseases and abortion in early pregnancies in female. This study was done to determine the effect of Natron and other Natron alternatives on the histology of kidney and liver in male Wistar Albino rats Twenty-five Wistar albino rats of the same sexes (male) weighing 100- 140 kg body weights were used and were divided into five groups. Group 1, 2, 3 and 4 were fed with feed mixed with natron powder, feed mixed with wood ash, feed mixed with sesame ash, feed mixed with palm fruit bunch ash respectively, while group 6 served as a normal control (fed with plain feed). The animals were kept in plastic cages, allowed to acclimatize for two weeks before the commencement of the experiment. They were fed with pelletized growers’ feed (Vital feed) and were allowed access to water. The rats were fed with 25kg of feeds for 12 days to allow them stabilized and recover from stress. Then the rats were fed with the pelletized feed for four weeks to all the groups. The animals were anaesthetized with chloroform vapour after 4 weeks and blood samples were collected through cardiac puncture into labeled tubes for electrolyte analyses. The labeled centrifuge tubes were allowed to stand for 30 minutes to clot and centrifuged at 4000 g for 10mm and the serum obtained was pipette into labeled tubes. The organs were subjected to histology. The liver tissues were embedded in paraffin wax, sectioned at 5μm and stained by hematoxylin and eosin. Proximate analysis showed that natron, palm bunch ash, sesame stem ash, and parkia wood ash had crude protein values of 0.03%, 0.05%, 0.07%, and 0.09% respectively. Fat and fiber were not present in both Natron and its alternatives. The appreciable amount of ash content in the food additives indicated good mineral preservation capacity. Parkia wood ash may contain toxic chemical substances that may affect the kidney and liver.
Keywords:
Natron; Kidney; Histology; Ash
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